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  • Featured Product
  • KD/KO Validated

RAB11A/B Polyclonal antibody

RAB11A/B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15903-1-AP

Synonyms

RAB11, Rab 11, RAB11A, Ras related protein Rab 11A, Rab-11

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse spleen tissue using RAB11A/B Polyclonal antibody (15903-1-AP)

    WB analysis of mouse spleen using 15903-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15903-1-AP (RAB11A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAB11A/B Polyclonal antibody (15903-1-AP)

    WB analysis of HeLa using 15903-1-AP

    WB result of RAB11A/B antibody (15903-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB11A/B transfected HeLa cells.

  • Western Blot (WB) analysis of MCF-7 cells using RAB11A/B Polyclonal antibody (15903-1-AP)

    WB analysis of MCF-7 using 15903-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 15903-1-AP (RAB11A/B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RAB11A/B Polyclonal antibody (15903-1-AP)

    WB analysis of Jurkat using 15903-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15903-1-AP (RAB11A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using RAB11A/B Polyclonal antibody (15903-1-AP)

    WB analysis of SH-SY5Y using 15903-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15903-1-AP (RAB11A/Bantibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using RAB11A/B Polyclonal antibody (15903-1-AP)

    IP experiment of Jurkat using 15903-1-AP

    IP result of anti-RAB11A/B (IP:15903-1-AP, 4ug; Detection:15903-1-AP 1:3000) with Jurkat cells lysate 2320 ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using RAB11A/B Polyclonal antibody (15903-1-AP)

    IHC staining of human heart using 15903-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15903-1-AP (RAB11A/B antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using RAB11A/B Polyclonal antibody (15903-1-AP)

    IHC staining of human heart using 15903-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15903-1-AP (RAB11A/B antibody at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RAB11A/B Polyclonal antibody (15903-1-AP)

    IF Staining of A549 using 15903-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RAB11A/B antibody (15903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RAB11A/B Polyclonal antibody (15903-1-AP)

    IF Staining of U2OS using 15903-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using RAB11A/B antibody (15903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RAB11A/B Polyclonal antibody (15903-1-AP)

    IF Staining of U2OS using 15903-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using RAB11A/B antibody (15903-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RAB11A/B Polyclonal antibody (15903-1-AP)

    IF Staining of NIH/3T3 using 15903-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 15903-1-AP (RAB11A/B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RAB11A/B Polyclonal antibody (15903-1-AP)

    IF Staining of HepG2 using 15903-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RAB11A/B antibody (15903-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse spleen tissue, Jurkat cells, MCF-7 cells, SH-SY5Y cells, HeLa cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15903-1-AP targets RAB11A/B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig, canine, monkey, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB11A/B fusion protein Ag8742 相同性解析による交差性が予測される生物種
Full Name RAB11A, member RAS oncogene family
Calculated molecular weight 216 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC013348
Gene symbol RAB11A
Gene ID (NCBI) 8766
RRIDAB_2173458
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB11 has two isoform genes RAB11A(YL8) and RAB11B(YPT3), and belongs to the small GTPase superfamily and Rab family. It regulates endocytic recycling. RAB11 may exert its functions by interacting with multiple effector proteins in different complexes. It acts as a major regulator of membrane delivery during cytokinesis. RAB11 regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. This antibody can recognize both RAB11A(24 kDa) and RAB11B(24 kDa).

Protocols

Product Specific Protocols
WB protocol for RAB11A/B antibody 15903-1-APDownload protocol
IHC protocol for RAB11A/B antibody 15903-1-APDownload protocol
IF protocol for RAB11A/B antibody 15903-1-APDownload protocol
IP protocol for RAB11A/B antibody 15903-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Nanomicro Lett

Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling

Authors - Junji Ren
View Article
humanIF

Nat Cell Biol

miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.

Authors - Cao Jingli J
View Article
canineIF

J Cell Biol

Septins promote macropinosome maturation and traffic to the lysosome by facilitating membrane fusion.

Authors - Lee Dolat
View Article
humanWB,CoIP

Cell Mol Life Sci

KIF13B mediates VEGFR2 recycling to modulate vascular permeability

Authors - Hyun-Dong Cho
View Article
humanIF

Oncogene

Piccolo mediates EGFR signaling and acts as a prognostic biomarker in esophageal squamous cell carcinoma.

Authors - W Zhang
View Article
humanWB,IF,CoIP

Clin Transl Med

ANXA6 suppresses the tumorigenesis of cervical cancer through autophagy induction.

Authors - Xin Sun
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Azita (Verified Customer) (06-16-2021)

Immunocytochemistry labelling of (4% PFA) fixed SH-SY5Y cell line that was pre-treated by the drug of interest and labelled by RAB11 polyclonal antibody at dilution of 1:100 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: SH-SY5Y cell line
FH

Stephen (Verified Customer) (09-12-2019)

Oc-2 cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours rab11 antibody incubated 1:300 overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) and Ph647 (red) to visualize DNA and F-actin respectively.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: mouse OC-2 cells
RAB11A/B Antibody Immunofluorescence, validation (1:300 dilution) in mouse OC-2 cells (Cat no:15903-1-AP)