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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite®594-conjugated RAB11A/B Polyclonal antibo (CL594-15903)

    IF Staining of A549 using CL594-15903

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®594 RAB11A/B antibody (CL594-15903) at dilution of 1:200.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

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  • KD/KO Validated

CoraLite®594-conjugated RAB11A/B Polyclonal antibody

RAB11A/B Polyclonal Antibody for IF/ICC
Cat No. CL594-15903

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

Rab 11, RAB11, RAB11A, Ras related protein Rab 11A, YL8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-15903 targets RAB11A/B in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB11A/B fusion protein Ag8742 相同性解析による交差性が予測される生物種
Full Name RAB11A, member RAS oncogene family
Calculated molecular weight 216 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC013348
Gene Symbol RAB11A
Gene ID (NCBI) 8766
RRIDAB_2919825
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62491
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB11 has two isoform genes RAB11A(YL8) and RAB11B(YPT3), and belongs to the small GTPase superfamily and Rab family. It regulates endocytic recycling. RAB11 may exert its functions by interacting with multiple effector proteins in different complexes. It acts as a major regulator of membrane delivery during cytokinesis. RAB11 regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. This antibody can recognize both RAB11A(24 kDa) and RAB11B(24 kDa).

Protocols

Product Specific Protocols
IF protocol for CL594 RAB11A/B antibody CL594-15903Download protocol
Standard Protocols
Click here to view our Standard Protocols