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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated RAB11A/B Polyclonal (CL488-15903)

    IF Staining of HepG2 using CL488-15903

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 RAB11A/B antibody (CL488-15903) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated RAB11A/B Polyclonal (CL488-15903)

    IF Staining of U2OS using CL488-15903

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 RAB11A/B antibody (CL488-15903) at dilution of 1:100.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated RAB11A/B Polyclonal antibody

RAB11A/B Polyclonal Antibody for IF/ICC
Cat No. CL488-15903

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

Rab 11, RAB11, RAB11A, Ras related protein Rab 11A, YL8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells, U2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15903 targets RAB11A/B in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB11A/B fusion protein Ag8742 相同性解析による交差性が予測される生物種
Full Name RAB11A, member RAS oncogene family
Calculated molecular weight 216 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC013348
Gene Symbol RAB11A
Gene ID (NCBI) 8766
RRIDAB_2919124
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62491
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB11 has two isoform genes RAB11A(YL8) and RAB11B(YPT3), and belongs to the small GTPase superfamily and Rab family. It regulates endocytic recycling. RAB11 may exert its functions by interacting with multiple effector proteins in different complexes. It acts as a major regulator of membrane delivery during cytokinesis. RAB11 regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. This antibody can recognize both RAB11A(24 kDa) and RAB11B(24 kDa).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RAB11A/B antibody CL488-15903Download protocol
Standard Protocols
Click here to view our Standard Protocols