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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RBM39 Monoclonal antibody

RBM39 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D2C8

Cat no : CL488-67420

Synonyms

HCC1, fSAP59, CC1.3, CAPERalpha, CAPER

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated RBM39 Monoclonal ant (CL488-67420)

    IF Staining of HepG2 using CL488-67420

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 RBM39 antibody (CL488-67420, Clone: 2D2C8 ) at dilution of 1:400.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated RBM39 Monoclonal ant (CL488-67420)

    FC experiment of HepG2 using CL488-67420

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RBM39 (CL488-67420, Clone:2D2C8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67420 targets RBM39 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RBM39 fusion protein Ag15766 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 39
Calculated molecular weight 530 aa, 59 kDa
Observed molecular weight 60-66 kDa
GenBank accession numberBC141835
Gene symbol RBM39
Gene ID (NCBI) 9584
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM39, also named as HCC1 or RNPC2, is a 530 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the splicing factor SR family. RBM39 is widely expressed in various tissues and with highly expression in pancreas, skeletal muscle, lung and brain. RBM39 is a transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1. RBM39 may be involved in pre-mRNA splicing process.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RBM39 antibody CL488-67420Download protocol
FC protocol for CL Plus 488 RBM39 antibody CL488-67420Download protocol
Standard Protocols
Click here to view our Standard Protocols