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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated RBM39 Monoclonal antibody (CL594-67420)

    IF Staining of HepG2 using CL594-67420

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 RBM39 antibody (CL594-67420, Clone: 2D2C8 ) at dilution of 1:500.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated RBM39 Monoclonal antibody (CL594-67420)

    FC experiment of HepG2 using CL594-67420

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RBM39 (CL594-67420, Clone:2D2C8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite®594-conjugated RBM39 Monoclonal antibody

RBM39 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67420
Clone No.2D2C8

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

HCC1, fSAP59, CC1.3, CAPERalpha, CAPER

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67420 targets RBM39 in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RBM39 fusion protein Ag15766 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 39
Calculated molecular weight 530 aa, 59 kDa
Observed molecular weight 60-66 kDa
GenBank accession numberBC141835
Gene Symbol RBM39
Gene ID (NCBI) 9584
RRIDAB_2934766
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14498
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM39, also named as HCC1 or RNPC2, is a 530 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the splicing factor SR family. RBM39 is widely expressed in various tissues and with highly expression in pancreas, skeletal muscle, lung and brain. RBM39 is a transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1. RBM39 may be involved in pre-mRNA splicing process.

Protocols

Product Specific Protocols
IF protocol for CL594 RBM39 antibody CL594-67420Download protocol
FC protocol for CL594 RBM39 antibody CL594-67420Download protocol
Standard Protocols
Click here to view our Standard Protocols