ChromoTek RFP-Booster Alexa Fluor® 568
anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins
Specificity
mCherry, mPlum, mRFP
Applications
IF, IHC, ICC
Conjugate
Alexa Fluor® 568
Host
Alpaca
Cat no : rb2AF568
Synonyms
Validation Data Gallery
Product Information
anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins
| Description | The RFP-Booster stabilizes, enhances, and reactivates the signal of RFP-fusion proteins such as mCherry, mRFP, etc.. Due to its small size, the RFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy: • Considerably higher tissue penetration rates • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments • Less than 2 nm epitope-label displacement minimizes linkage error • Monovalent VHHs do not cluster their epitopes • Validation: structure and function characterized • Consistent and reliable performance due to recombinant production |
| Applications | IF, IHC, ICC |
| Host | Alpaca |
| Specificity/Target | mCherry, mPlum, mRFP, mRFPruby, mKate2 For the complete list, please click here: Fluorescent protein specificity table Nano-Booster |
| Conjugate | Alexa Fluor® 568 |
| Physical State | Liquid |
| Suggested Dilution | IF/ICC/IHC: 1:500- 1:1,000 |
| Type | Nanobody |
| Class | Recombinant |
| DOL | 2 fluorophores per Nanobody |
| RRID | AB_2827576 |
| Affinity (KD) | Dissociation constant KD of 5nM |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA Preservative: 0.09% Sodium azide |
| Storage Condition | Shipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light. |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
| SDS |
|---|
| SDS RFP-Booster Alexa Fluor 568 (PDF) |
| Protocol |
|---|
| Protocol RFP-Booster Alexa Fluor 568 (PDF) |
| RFP-TRAP Specificity |
|---|
| Fluorescent protein specificity table Nano-Booster |
| Brochure |
|---|
| Chromotek nanobodies brochure (PDF) |
Publications
| Application | Title |
|---|---|
Dev Cell Identification of a core transcriptional program driving the human renal mesenchymal-to-epithelial transition | |
EMBO J PUFFFIN: an ultra-bright, customisable, single-plasmid system for labelling cell neighbourhoods | |
Dev Cell Mauve/LYST limits fusion of lysosome-related organelles and promotes centrosomal recruitment of microtubule nucleating proteins. | |
Ecotoxicol Environ Saf Chronic exposure to low-dose deltamethrin can lead to colon tissue injury through PRDX1 inactivation-induced mitochondrial oxidative stress injury and gut microbial dysbiosis |