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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated RIC8A Monoclonal ant (CL488-66625)

    IF Staining of mouse brain using CL488-66625

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 RIC8A antibody (CL488-66625, Clone: 1E3B11 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated RIC8A Monoclonal ant (CL488-66625)

    IF Staining of mouse brain using CL488-66625

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 RIC8A antibody (CL488-66625, Clone: 1E3B11 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated RIC8A Monoclonal antibody

RIC8A Monoclonal Antibody for IF-P
Cat No. CL488-66625
Clone No.1E3B11

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

IF-P

Synonyms

Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66625 targets RIC8A in IF-P applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RIC8A fusion protein Ag1593 相同性解析による交差性が予測される生物種
Full Name resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
Calculated molecular weight 60 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC011821
Gene Symbol RIC8A
Gene ID (NCBI) 60626
RRIDAB_2919352
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NPQ8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RIC8A antibody CL488-66625Download protocol
Standard Protocols
Click here to view our Standard Protocols