CoraLite®594-conjugated RIC8A Monoclonal antibody
RIC8A Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, Mouse
Applications
IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1E3B11
Cat no : CL594-66625
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-66625 targets RIC8A in IF-P applications and shows reactivity with Human, Rat, Mouse samples.
Tested Reactivity | Human, Rat, Mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | RIC8A fusion protein Ag1593 相同性解析による交差性が予測される生物種 |
Full Name | resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) |
Calculated molecular weight | 60 kDa |
Observed molecular weight | 65 kDa |
GenBank accession number | BC011821 |
Gene symbol | RIC8A |
Gene ID (NCBI) | 60626 |
RRID | AB_2920016 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 RIC8A antibody CL594-66625 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |