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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated FSP1/S100A4 Polyclonal antibody

FSP1/S100A4 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-16105

Synonyms

S100A4, FSP1, Fibroblast-specific protein-1, Fibroblast-specific protein 1, Fibroblast Specific Protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated S100A4 Polyclonal antibody (CL594-16105)

    IF Staining of HeLa using CL594-16105

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 S100A4 antibody (CL594-16105) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite®594-conjugated S100A4 Polyclonal antibody (CL594-16105)

    FC experiment of Jurkat using CL594-16105

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human S100A4 (CL594-16105) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated S100A4 Polyclonal antibody (CL594-16105)

    FC experiment of HeLa using CL594-16105

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human S100A4 (CL594-16105) (red), or 0.4 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000, Clone: ) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells, Jurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-16105 targets FSP1/S100A4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FSP1/S100A4 fusion protein Ag9019 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A4
Calculated molecular weight 101 aa, 12 kDa
Observed molecular weight 10-12 kDa
GenBank accession numberBC016300
Gene symbol S100A4
Gene ID (NCBI) 6275
RRIDAB_2919828
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

Protocols

Product Specific Protocols
IF protocol for CL594 FSP1/S100A4 antibody CL594-16105Download protocol
Standard Protocols
Click here to view our Standard Protocols