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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Biotin Plus-conjugated STXBP1 Monoclonal antibody

STXBP1 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IHC

Conjugate

Biotin Plus

CloneNo.

1B5B3

Cat no : Biotin-67137

Synonyms

N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using Biotin Plus-conjugated STXBP1 Monoclonal antibody (Biotin-67137)

    IHC staining of mouse brain using Biotin-67137

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-67137 (STXBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Biotin Plus-conjugated STXBP1 Monoclonal antibody (Biotin-67137)

    IHC staining of mouse brain using Biotin-67137

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-67137 (STXBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-67137 targets STXBP1 in IHC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen STXBP1 fusion protein Ag28605 相同性解析による交差性が予測される生物種
Full Name syntaxin binding protein 1
Calculated molecular weight 594 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC015749
Gene symbol STXBP1
Gene ID (NCBI) 6812
Conjugate Biotin Plus
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.

Protocols

Product Specific Protocols
IHC protocol for Biotin Plus STXBP1 antibody Biotin-67137Download protocol
Standard Protocols
Click here to view our Standard Protocols