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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated STXBP1 Monoclonal antibody

STXBP1 Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B5B3

Cat no : CL488-67137

Synonyms

N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated STXBP1 Monoclonal an (CL488-67137)

    WB analysis using CL488-67137

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67137 (STXBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated STXBP1 Monoclonal an (CL488-67137)

    FC experiment of HeLa using CL488-67137

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human STXBP1 (CL488-67137, Clone:1B5B3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67137 targets STXBP1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen STXBP1 fusion protein Ag28605 相同性解析による交差性が予測される生物種
Full Name syntaxin binding protein 1
Calculated molecular weight 594 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC015749
Gene symbol STXBP1
Gene ID (NCBI) 6812
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 STXBP1 antibody CL488-67137Download protocol
FC protocol for CL Plus 488 STXBP1 antibody CL488-67137Download protocol
Standard Protocols
Click here to view our Standard Protocols