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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated TSG101 Monoclonal antibody (CL594-67381)

    IF Staining of HeLa using CL594-67381

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 TSG101 antibody (CL594-67381, Clone: 2B7G8 ) at dilution of 1:200.

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CoraLite®594-conjugated TSG101 Monoclonal antibody

TSG101 Monoclonal Antibody for IF/ICC
Cat No. CL594-67381
Clone No.2B7G8

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Synonyms

ESCRT I complex subunit TSG101, TSG10, TSG101, tumor susceptibility gene 101, VPS23

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67381 targets TSG101 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TSG101 fusion protein Ag28569 相同性解析による交差性が予測される生物種
Full Name tumor susceptibility gene 101
Calculated molecular weight 44 kDa
Observed molecular weight 43-46 kDa
GenBank accession numberBC002487
Gene Symbol TSG101
Gene ID (NCBI) 7251
RRIDAB_3084763
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99816
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Protocols

Product Specific Protocols
IF protocol for CL594 TSG101 antibody CL594-67381Download protocol
Standard Protocols
Click here to view our Standard Protocols