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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated TSG101 Monoclonal antibody

TSG101 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2B7G8

Cat no : CL647-67381

Synonyms

ESCRT I complex subunit TSG101, TSG10, TSG101, tumor susceptibility gene 101, VPS23

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL647-67381

    FC experiment of HeLa using CL647-67381

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human TSG101 (CL647-67381, Clone:2B7G8) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TSG101 Ready-To-Use IHC Kit. TSG101 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67381-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL647-67381 targets TSG101 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TSG101 fusion protein Ag28569 相同性解析による交差性が予測される生物種
Full Name tumor susceptibility gene 101
Calculated molecular weight 44 kDa
Observed molecular weight 43-46 kDa
GenBank accession numberBC002487
Gene symbol TSG101
Gene ID (NCBI) 7251
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 TSG101 antibody CL647-67381Download protocol
Standard Protocols
Click here to view our Standard Protocols