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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated UCH-L1/PGP9.5 Monoclonal antibody

UCH-L1/PGP9.5 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C9E11

Cat no : CL488-66230

Synonyms

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated UCH-L1/PGP9.5 Monocl (CL488-66230)

    IF Staining of mouse brain using CL488-66230

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 UCHL1/PGP9.5 antibody (CL488-66230, Clone: 1C9E11 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 488-conjugated UCHL1/PGP9.5 Monoclo (CL488-66230)

    IF Staining of SH-SY5Y using CL488-66230

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 UCHL1/PGP9.5 antibody (CL488-66230, Clone: 1C9E11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Y79 cells using CoraLite® Plus 488-conjugated UCHL1/PGP9.5 Monoclo (CL488-66230)

    FC experiment of Y79 using CL488-66230

    1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human UCHL1/PGP9.5 (CL488-66230, Clone:1C9E11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inY79 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66230 targets UCH-L1/PGP9.5 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UCH-L1/PGP9.5 fusion protein Ag6547 相同性解析による交差性が予測される生物種
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Calculated molecular weight 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC000332
Gene symbol UCHL1
Gene ID (NCBI) 7345
RRIDAB_2883290
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 UCH-L1/PGP9.5 antibody CL488-66230Download protocol
Standard Protocols
Click here to view our Standard Protocols