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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated UCH-L1/PGP9.5 Monoclonal antibody

UCH-L1/PGP9.5 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1C9E11

Cat no : CL647-66230

Synonyms

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 647-conjugated UCH-L1/PGP9.5 Monocl (CL647-66230)

    IF Staining of SH-SY5Y using CL647-66230

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 647 UCHL1/PGP9.5 antibody (CL647-66230, Clone: 1C9E11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite® Plus 647-conjugated UCHL1/PGP9.5 Monoclo (CL647-66230)

    FC experiment of SH-SY5Y using CL647-66230

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated UCHL1/PGP9.5 Monoclonal antibody (CL647-66230, Clone:1C9E11)(red), or 0.8 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66230 targets UCH-L1/PGP9.5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UCH-L1/PGP9.5 fusion protein Ag6547 相同性解析による交差性が予測される生物種
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Calculated molecular weight 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC000332
Gene symbol UCHL1
Gene ID (NCBI) 7345
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitin C-terminal hydrolase L1 (UCHL1) was originally identified as a neuronal protein that accounts for nearly 2% of total brain proteins. UCHL1 activity protects neurons from hypoxic injury, and binding of stroke-induced reactive lipid species to the cysteine 152 (C152) of UCHL1 unfolds the protein and disrupts its function. Reduced hydrolytic activity of mutant UCHL1 is implicated in the pathophysiologic process of Parkinson's and Alzheimer's disease due to abnormal neurotoxic protein aggregation. (PMID: 31356902, PMID: 30760601)

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 UCH-L1/PGP9.5 antibody CL647-66230Download protocol
Standard Protocols
Click here to view our Standard Protocols