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CoraLite® Plus 750-conjugated YY1 Monoclonal antibody

YY1 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2E11C5

Cat no : CL750-66281

Synonyms

DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor


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Tested Applications

Positive WB detected inNIH/3T3 cells, HepG2 cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-66281 targets YY1 in WB applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen YY1 fusion protein Ag17732 相同性解析による交差性が予測される生物種
Full Name YY1 transcription factor
Calculated molecular weight 414 aa, 45 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC037308
Gene symbol YY1
Gene ID (NCBI) 7528
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 YY1 antibody CL750-66281Download protocol
Standard Protocols
Click here to view our Standard Protocols