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CoraLite® Plus 750-conjugated YY1 Monoclonal antibody
YY1 Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
2E11C5
Cat no : CL750-66281
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | NIH/3T3 cells, HepG2 cells, HUVEC cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL750-66281 targets YY1 in WB applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | YY1 fusion protein Ag17732 相同性解析による交差性が予測される生物種 |
Full Name | YY1 transcription factor |
Calculated molecular weight | 414 aa, 45 kDa |
Observed molecular weight | 65-70 kDa |
GenBank accession number | BC037308 |
Gene symbol | YY1 |
Gene ID (NCBI) | 7528 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 750 YY1 antibody CL750-66281 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |