CoraLite® Plus 488-conjugated Acetyl-Tubulin (Lys40) Monoclonal antibody
Acetyl-Tubulin (Lys40) Monoclonal Antibody for IF/ICC, IF-Fro
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig, canine
Applications
IF/ICC, IF-Fro
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
7E5H8
Cat no : CL488-66200
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-Fro detected in | mouse kidney tissue |
| Positive IF/ICC detected in | MDCK cells, hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 3 publications below |
Product Information
CL488-66200 targets Acetyl-Tubulin (Lys40) in IF/ICC, IF-Fro applications and shows reactivity with human, mouse, rat, pig, canine samples.
| Tested Reactivity | human, mouse, rat, pig, canine |
| Cited Reactivity | human, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | tubulin, alpha 1a |
| Calculated molecular weight | 52 kDa |
| GenBank accession number | NM_006009 |
| Gene symbol | Alpha Tubulin |
| Gene ID (NCBI) | 7846 |
| RRID | AB_2883282 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549). This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Acetyl-Tubulin (Lys40) antibody CL488-66200 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia | ||
STAR Protoc Isolation, expansion, differentiation, and histological processing of human nasal epithelial cells | ||
BBA Adv The dynamic nature of the coronavirus receptor, angiotensin-converting enzyme 2 (ACE2) in differentiating airway epithelia. |
