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acetylated Tubulin(Lys40) 抗体 47 文献

Mouse Monoclonal| カタログ番号: 66200-1-Ig |クローン番号: 7E5H8

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Related Primary Antibodies

適用アプリケーション

アプリケーション:
WB, IHC, IF, ELISA
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Images See all

acetylated Tubulin(Lys40) Antibody WB rat testis tissue 66200-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66200-1-Ig (acetylated Tubulin(Lys40) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

acetylated Tubulin(Lys40) Antibody IF MDCK cells 66200-1-Ig

Immunofluorescent images of MDCK cells stained for IFT88 rabbit pAb (13967-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 13967-1-AP, and Alexa Fluor 488-conjugated AffiniPure Goat anti-Mouse IgG (H+L) for 66200-1-Ig.

acetylated Tubulin(Lys40) Antibody IHC mouse ovary tissue 66200-1-Ig

Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 66200-1-Ig (acetylated Tubulin(Lys40) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

種特異性:
human, mouse, rat, Canine, pig, bovine, Drosophila
WB ポジティブ:
rat testis tissue, NCCIT cells, Neuro-2a cells, L02 cells, pig cerebellum tissue, rat brain tissue, mouse ovary tissue, HEK-293 cells, mouse testis
IHC ポジティブ:
mouse ovary tissue, human gliomas tissue, rat brain tissue, mouse brain tissue, human lung cancer tissue
IF ポジティブ:
MDCK cells, mouse brain tissue
推奨希釈倍率:
WB : 1:2000-1:10000
IHC : 1:1000-1:4000
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Mouse
精製方法:
Protein G purification
アイソタイプ:
IgG1
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
Peptide
正式名:
tubulin, alpha 1a
計算分子量:
52 kDa
検出分子量:
50-55 kDa
GenBank accession number:
NM_006009
Gene ID (NCBI):
7846
遺伝子シンボル:
TUBA1A
別名:
Ac tubulin, acetylated alpha tubulin, Acetylated tubulin, Acetylated tubulin (Lys40), acetylated-a-tubulin, acetyl-a-tubulin, Ac-tubulin, alpha tubulin, Alpha tubulin 3, tubulin

背景説明

Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549)


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