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KD/KO Validated

CoraLite® Plus 488-conjugated Alpha Tubulin Monoclonal antibody

Name: CoraLite® Plus 488-conjugated Alpha Tubulin Monoclonal antibody
Brand: Proteintech
SKU: CL488-66031

Alpha Tubulin Monoclonal Antibody for WB, IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, canine

Applications

WB, IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

CloneNo.

1E4C11

Review Product

Cat no : CL488-66031

Synonyms

TUBA1B, Tubulin alpha 1B chain, Tubulin alpha, EC:3.6.5.-, Detyrosinated tubulin alpha-1B chain

Validation Data Gallery

Filter:

AllWBIF/ICCIFFC (Intra)

WB analysis using CL488-66031
Various lysates were subjected to SDS PAGE followed by western blot with CL488-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using CL488-66031
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66031 (alpha Tubulin antibody), at dilution of 1:100.
IF Staining of mouse embryo using CL488-66031
E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram
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E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram
IF Staining of mouse embryo using CL488-66031
E14.5 FFPE mouse embryo section stained for E-cadherin (red, Cat. No 20874-1-AP) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see the epithelial layer of tissue clearly stained, particularly the skin, eyes, and respiratory system. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram
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E14.5 FFPE mouse embryo section stained for E-cadherin (red, Cat. No 20874-1-AP) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see the epithelial layer of tissue clearly stained, particularly the skin, eyes, and respiratory system. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram
FC experiment of HeLa using CL488-66031
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Alpha Tubulin (CL488-66031, Clone:1E4C11) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. CL488-66031

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected in	HeLa cells, mouse embryo tissue
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:300-1:600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IF	See 4 publications below

Product Information

CL488-66031 targets Alpha Tubulin in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity	human, mouse, rat, canine
Cited Reactivity	human, mouse
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Alpha Tubulin fusion protein Ag18034 相同性解析による交差性が予測される生物種
Full Name	tubulin, alpha 1b
Calculated molecular weight	50 kDa
Observed molecular weight	50-55 kDa
GenBank accession number	BC009314
Gene symbol	Alpha Tubulin
Gene ID (NCBI)	10376
RRID	AB_2883232
Conjugate	CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths	493 nm / 522 nm
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage Conditions	Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 Alpha Tubulin antibody CL488-66031	Download protocol
IF protocol for CL Plus 488 Alpha Tubulin antibody CL488-66031	Download protocol
FC protocol for CL Plus 488 Alpha Tubulin antibody CL488-66031	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	IF	Free Radic Biol Med Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling Authors - Yaoqiu Wu View Article
		Clin Genet A novel homozygous CFAP65 mutation in humans causes male infertility with multiple morphological abnormalities of the sperm flagella. Authors - Xueguang Zhang View Article
human	IF	Transl Androl Urol Novel bi-allelic mutations in DNAH1 cause multiple morphological abnormalities of the sperm flagella resulting in male infertility. Authors - Chuan Jiang View Article
mouse	IF	Reprod Toxicol Bisphenol M inhibits mouse oocyte maturation in vitro by disrupting cytoskeleton architecture and cell cycle processes Authors - Huilei Chen View Article
mouse	IF	Am J Physiol Cell Physiol Melatonin partially rescues defects induced by tranexamic acid exposure during oocyte maturation in mice Authors - Fei Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Alpha Tubulin Monoclonal antibody

Alpha Tubulin Monoclonal Antibody for WB, IF/ICC, FC (Intra)

Validation Data Gallery

WB analysis using CL488-66031

IF Staining of HeLa using CL488-66031

IF Staining of mouse embryo using CL488-66031

IF Staining of mouse embryo using CL488-66031

FC experiment of HeLa using CL488-66031

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling

A novel homozygous CFAP65 mutation in humans causes male infertility with multiple morphological abnormalities of the sperm flagella.

Novel bi-allelic mutations in DNAH1 cause multiple morphological abnormalities of the sperm flagella resulting in male infertility.

Bisphenol M inhibits mouse oocyte maturation in vitro by disrupting cytoskeleton architecture and cell cycle processes

Melatonin partially rescues defects induced by tranexamic acid exposure during oocyte maturation in mice

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