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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Alpha Tubulin Monoclonal antibody

Alpha Tubulin Monoclonal Antibody for WB, IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, canine

Applications

WB, IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E4C11

Cat no : CL594-66031

Synonyms

TUBA1B, Tubulin alpha 1B chain, Tubulin alpha, EC:3.6.5.-, Detyrosinated tubulin alpha-1B chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite®594-conjugated Alpha Tubulin Monoclonal a (CL594-66031)

    WB analysis using CL594-66031

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated Alpha Tubulin Monoclonal a (CL594-66031)

    IF Staining of HepG2 using CL594-66031

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66031 (alpha Tubulin antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Alpha Tubulin Monoclonal a (CL594-66031)

    IF Staining of HeLa using CL594-66031

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®594-conjugated version of this antibody, CL594-66031 (alpha Tubulin antibody), at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated Alpha Tubulin Monoclonal a (CL594-66031)

    IF Staining of HepG2 using CL594-66031

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66031 (alpha Tubulin antibody) at dilution of 1:50.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated Alpha Tubulin Monoclonal a (CL594-66031)

    FC experiment of HepG2 using CL594-66031

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Alpha Tubulin (CL594-66031, Clone:1E4C11) (red), or 0.8 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 3 publications below

Product Information

CL594-66031 targets Alpha Tubulin in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha Tubulin fusion protein Ag18034 相同性解析による交差性が予測される生物種
Full Name tubulin, alpha 1b
Calculated molecular weight 50 kDa
GenBank accession numberBC009314
Gene symbol Alpha Tubulin
Gene ID (NCBI) 10376
RRIDAB_2883483
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Protocols

Product Specific Protocols
WB protocol for CL594 Alpha Tubulin antibody CL594-66031Download protocol
IF protocol for CL594 Alpha Tubulin antibody CL594-66031Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Exp Mol Med

Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes.

Authors - Long-Fei Wu
View Article
mouseIF

Biology (Basel)

Effects of IGF-1 on the Three-Dimensional Culture of Ovarian Preantral Follicles and Superovulation Rates in Mice.

Authors - Shizhen Dai
View Article
mouseIF

Int J Mol Sci

Overexpression of Tfap2a in Mouse Oocytes Impaired Spindle and Chromosome Organization

Authors - Juan Lin
View Article