CoraLite® Plus 488-conjugated c-SRC Monoclonal antibody
c-SRC Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human, hamster, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5E10C4
Cat no : CL488-60315
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-60315 targets c-SRC in FC (Intra) applications and shows reactivity with human, hamster, mouse samples.
| Tested Reactivity | human, hamster, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | c-SRC fusion protein Ag22306 相同性解析による交差性が予測される生物種 |
| Full Name | v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC011566 |
| Gene symbol | SRC |
| Gene ID (NCBI) | 6714 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 c-SRC antibody CL488-60315 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
