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CoraLite®594-conjugated c-SRC Monoclonal antibody

c-SRC Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-60315
Clone No.5E10C4

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, hamster

Applications

IF/ICC, FC (Intra)

SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL594-60315 targets c-SRC in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, hamster samples.

Tested Reactivity human, mouse, hamster
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen c-SRC fusion protein Ag22306 相同性解析による交差性が予測される生物種
Full Name v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC011566
Gene Symbol SRC
Gene ID (NCBI) 6714
ENSEMBL Gene IDENSG00000197122
RRIDAB_2883461
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12931
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

Protocols

Product Specific Protocols
IF protocol for CL594 c-SRC antibody CL594-60315Download protocol
FC protocol for CL594 c-SRC antibody CL594-60315Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Cancer

Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression.

Authors - Zhenzhen Pan
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