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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated c-SRC Monoclonal antibody

c-SRC Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, hamster

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5E10C4

Cat no : CL594-60315

Synonyms

SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite®594-conjugated c-SRC Monoclonal antibody (CL594-60315)

    IF Staining of human breast cancer using CL594-60315

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594-conjugated c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated c-SRC Monoclonal antibody (CL594-60315)

    IF Staining of MCF-7 using CL594-60315

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CL594-60315 (c-SRC antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated c-SRC Monoclonal antibody (CL594-60315)

    IF Staining of MCF-7 using CL594-60315

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated c-SRC Monoclonal antibody (CL594-60315)

    IF Staining of MCF-7 using CL594-60315

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated c-SRC Monoclonal antibody (CL594-60315)

    IF Staining of MCF-7 using CL594-60315

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®594-conjugated c-SRC Monoclonal antibody (CL594-60315)

    FC experiment of MCF-7 using CL594-60315

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human c-SRC (CL594-60315, Clone:5E10C4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL594-60315 targets c-SRC in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, hamster samples.

Tested Reactivity human, mouse, hamster
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen c-SRC fusion protein Ag22306 相同性解析による交差性が予測される生物種
Full Name v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC011566
Gene symbol SRC
Gene ID (NCBI) 6714
RRIDAB_2883461
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

Protocols

Product Specific Protocols
IF protocol for CL594 c-SRC antibody CL594-60315Download protocol
FC protocol for CL594 c-SRC antibody CL594-60315Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Cancer

Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression.

Authors - Zhenzhen Pan
View Article