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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

p504S,AMACR Monoclonal antibody

p504S,AMACR Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H9B5

Cat no : 60240-1-Ig

Synonyms

AMACR, p504S, EC:5.1.99.4, AMACR/p504S, Alpha-methylacyl-CoA racemase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using p504S,AMACR Monoclonal antibody (60240-1-Ig)

    WB analysis using 60240-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using p504S,AMACR Monoclonal antibody (60240-1-Ig)

    WB analysis using 60240-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using p504S,AMACR Monoclonal antibody (60240-1-Ig)

    WB analysis of HEK-293 using 60240-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using p504S,AMACR Monoclonal antibody (60240-1-Ig)

    IF Staining of A431 using 60240-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using p504S,AMACR antibody (60240-1-Ig, Clone: 2H9B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

Positive WB detected inHepG2 cells, PC-3 cells, HEK-293 cells, HeLa cells, LNCaP cells, HSC-T6 cells, 4T1 cells, NIH/3T3 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60240-1-Ig targets p504S,AMACR in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen p504S,AMACR fusion protein Ag8720 相同性解析による交差性が予測される生物種
Full Name alpha-methylacyl-CoA racemase
Calculated molecular weight 382 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC009471
Gene symbol AMACR
Gene ID (NCBI) 23600
RRIDAB_2881363
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing. Defects in AMACR are the cause of alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).

Protocols

Product Specific Protocols
WB protocol for p504S,AMACR antibody 60240-1-IgDownload protocol
IF protocol for p504S,AMACR antibody 60240-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols