CoraLite® Plus 488-conjugated p504S,AMACR Monoclonal antibody
p504S,AMACR Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2H9B5
Cat no : CL488-60240
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | LNCaP cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-60240 targets p504S,AMACR in FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | p504S,AMACR fusion protein Ag8720 相同性解析による交差性が予測される生物種 |
| Full Name | alpha-methylacyl-CoA racemase |
| Calculated molecular weight | 382 aa, 42 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC009471 |
| Gene symbol | AMACR |
| Gene ID (NCBI) | 23600 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AMACR(Alpha-methylacyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing. Defects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 p504S,AMACR antibody CL488-60240 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
