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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated p504S,AMACR Monoclonal antibody

p504S,AMACR Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H9B5

Cat no : CL488-60240

Synonyms

AMACR, p504S, EC:5.1.99.4, AMACR/p504S, Alpha-methylacyl-CoA racemase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated p504S,AMACR Monoclon (CL488-60240)

    IF Staining of A431 using CL488-60240

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 p504S,AMACR antibody (CL488-60240, Clone: 2H9B5 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of LNCaP cells using CoraLite® Plus 488-conjugated p504S,AMACR Monoclon (CL488-60240)

    FC experiment of LNCaP using CL488-60240

    1X10^6 LNCaP cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human p504S,AMACR (CL488-60240, Clone:2H9B5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inLNCaP cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60240 targets p504S,AMACR in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen p504S,AMACR fusion protein Ag8720 相同性解析による交差性が予測される生物種
Full Name alpha-methylacyl-CoA racemase
Calculated molecular weight 382 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC009471
Gene symbol AMACR
Gene ID (NCBI) 23600
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AMACR(Alpha-methylacyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing. Defects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 p504S,AMACR antibody CL488-60240Download protocol
Standard Protocols
Click here to view our Standard Protocols