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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated smooth muscle actin specific Recombinant antibody

smooth muscle actin specific Recombinant Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, chicken

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5H7

Cat no : CL594-80008

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Actin, aortic smooth muscle, intermediate form

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated smooth muscle actin specif (CL594-80008)

    IF Staining of mouse heart using CL594-80008

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using CoraLite®594-conjugated smooth muscle actin specif (CL594-80008)

    IF Staining of H9C2 using CL594-80008

    Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of C2C12 cells using CoraLite®594-conjugated smooth muscle actin specif (CL594-80008)

    FC experiment of C2C12 using CL594-80008

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human smooth muscle actin specific (CL594-80008, Clone:5H7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inH9C2 cells
Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-80008 targets smooth muscle actin specific in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, chicken samples.

Tested Reactivity human, mouse, rat, pig, chicken
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name actin, alpha 2, smooth muscle, aorta
Calculated molecular weight 42 kDa
Observed molecular weight42 kDa
GenBank accession numberNM_001613
Gene symbol Alpha smooth muscle actin
Gene ID (NCBI) 59
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Protocols

Product Specific Protocols
IF protocol for CL594 smooth muscle actin specific antibody CL594-80008Download protocol
FC protocol for CL594 smooth muscle actin specific antibody CL594-80008Download protocol
Standard Protocols
Click here to view our Standard Protocols