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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated smooth muscle actin specific Recombinant antibody

smooth muscle actin specific Recombinant Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human, rat, chicken, pig, zebrafish

Applications

IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

5H7

Cat no : CL647-80008

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using CoraLite® Plus 647-conjugated smooth muscle actin (CL647-80008)

    IF Staining of mouse small intestine using CL647-80008

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7 ) at dilution of 1:200, ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), ASCT2 antibody (20350-1-AP, green).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using CoraLite® Plus 647-conjugated smooth muscle actin (CL647-80008)

    IF Staining of mouse small intestine using CL647-80008

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7 ) at dilution of 1:200, ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), ASCT2 antibody (20350-1-AP, green).

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Tested Applications

Positive IF-P detected inmouse small intestine tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-80008 targets smooth muscle actin specific in IF-P applications and shows reactivity with mouse, human, rat, chicken, pig, zebrafish samples.

Tested Reactivity mouse, human, rat, chicken, pig, zebrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name actin, alpha 2, smooth muscle, aorta
Calculated molecular weight 42 kDa
GenBank accession numberNM_001613
Gene symbol Alpha smooth muscle actin
Gene ID (NCBI) 59
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 smooth muscle actin specific antibody CL647-80008Download protocol
Standard Protocols
Click here to view our Standard Protocols