Untreated, and etoposide (60uM, 5h) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5/GSDME antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB result of DFNA5/ GSDME antibody (13075-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DFNA5/ GSDME transfected A549 cells.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-DFNA5/GSDME (IP:13075-1-AP, 4ug; Detection:13075-1-AP 1:15000) with SH-SY5Y cells lysate 1240 ug.
1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human DFNA5/GSDME (13075-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
