Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-Lamin B1 (IP:12987-1-AP, 4ug; Detection:12987-1-AP 1:20000) with HeLa cells lysate 1560 ug.
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Lamin B1 (12987-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin B1 antibody (12987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
