Various lysates were subjected to SDS PAGE followed by western blot with 21773-1-AP (ZO-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP result of anti-ZO-1 (IP:21773-1-AP, 4ug; Detection:21773-1-AP 1:500) with HEK-293 cells lysate 2320 ug.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21773-1-AP (ZO-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21773-1-AP (ZO-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (21773-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).
1X10^6 MCF-7 cells were stained with 0.2ug ZO-1 antibody (21773-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
