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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis using 66013-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of Calu-1 cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of Calu-1 using 66013-1-Ig

    WB result of HLA class I ABC antibody (66013-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HLA class I ABC transfected Calu-1 cells.

  • Western Blot (WB) analysis of U-937 cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of U-937 using 66013-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of Raji using 66013-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of HeLa using 66013-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of HepG2 using 66013-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis using 66013-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human white blood using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of human white blood using 66013-1-Ig

    human white blood cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig spleen tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of pig spleen using 66013-1-Ig

    pig spleen tissue was subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of NCCIT using 66013-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IP experiment of HepG2 using 66013-1-Ig

    IP result of anti-HLA class I ABC (IP:66013-1-Ig, 4ug; Detection:66013-1-Ig 1:4000) with HepG2 cells lysate 2000 ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human tonsillitis using 66013-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human tonsillitis using 66013-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human liver cancer using 66013-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human stomach cancer using 66013-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human stomach cancer using 66013-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human liver cancer using 66013-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IF Staining of human tonsillitis using 66013-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using HLA class I ABC antibody (66013-1-Ig, Clone: 5C5B7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IF Staining of Raji using 66013-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA class I ABC antibody (66013-1-Ig, Clone: 5C5B7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of Raji cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    FC experiment of Raji using 66013-1-Ig

    1X10^6 Raji cells were surface stained with 0.4 ug Anti-Human HLA class I ABC (66013-1-Ig, Clone:5C5B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 66013-1-Ig

    Affinity and Kinetic Characterization of 66013-1-Ig

    Biolayer interferometry (BLl) kinetic assays of 66013-2-Ig against Human HLA class I (HLA-A) were performed. The affinity constant is 1.03 nM.

  • Featured Product
  • KD/KO Validated

HLA class I ABC Monoclonal antibody

HLA class I ABC Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC, IP, ELISA
Cat No. 66013-1-Ig
Clone No.5C5B7
Publications(23)

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IF-P, FC, IP, ELISA

Synonyms

HLA-A, 5C5B7, HLA A, HLA class I, HLA class I (HLA-A)

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, U-937 cells, Raji cells, human white blood, NCCIT cells, pig spleen tissue, Calu-1 cells, HEK-293 cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inRaji cells
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:10000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66013-1-Ig targets HLA class I ABC in WB, IHC, IF/ICC, IF-P, FC, IP, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7488

Product name: Recombinant human HLA class I (HLA-A) protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 29-302 aa of BC003069

Sequence: MRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQKMEPRAPWIEQEGPEYWDQETRNMKAHSQTDRANLGTLRGYYNQSEDGSHTIQIMYGCDVGPDGRFLRGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAVHAAEQRRVYLEGRCVDGLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWELSS

相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, A
Calculated molecular weight 41 kDa
Observed molecular weight 40-44 kDa
GenBank accession numberBC003069
Gene Symbol HLA-A
Gene ID (NCBI) 3105
RRIDAB_11042593
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP04439
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Protocols

Product Specific Protocols
FC protocol for HLA class I ABC antibody 66013-1-IgDownload protocol
IF protocol for HLA class I ABC antibody 66013-1-IgDownload protocol
IHC protocol for HLA class I ABC antibody 66013-1-IgDownload protocol
IP protocol for HLA class I ABC antibody 66013-1-IgDownload protocol
WB protocol for HLA class I ABC antibody 66013-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Mol Cancer

IFITM3 enhances immunosensitivity via MHC-I regulation and is associated with the efficacy of anti-PD-1/-L1 therapy in SCLC.

Authors - Yanan Cui
View Article

Adv Sci (Weinh)

Engineered Xenogeneic Bone Scaffold with IL-10 Nanodelivery System: Immunomodulation and BMSC Fate Programming for Skull Defect Repair.

Authors - Weihao Lv
View Article

Adv Sci (Weinh)

Genetic Ablation and Multi-Omics Profiling Reveal CEP55 as a Key Driver of Tumorigenesis in Diverse Cancer Models.

Authors - Behnam Rashidieh
View Article
FC

Cell Death Dis

AZIN1-dependent polyamine synthesis accelerates tumor cell cycle progression and impairs effector T-cell function in osteosarcoma

Authors - Jiaming Yu
View Article
IHC

Int J Biol Sci

Prolonged DEHP exposure enhances the stemness and metastatic potential of TNBC cells in an MSI2-dependent manner

Authors - Mahendra Jadhao
View Article
humanWB,IP

Proc Natl Acad Sci U S A

Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteins

Authors - Fengwen Zhang
View Article
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