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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 750 Anti-Mouse CD103 (2E7)

CD103 Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2E7

Cat no : CL750-65047

Synonyms

A530055J10, alpha E1, alpha M290, aM290, CD103, Itgae

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using CoraLite® Plus 750 Anti-Mouse CD103 (2E7) (CL750-65047)

    FC experiment of mouse splenocytes using CL750-65047

    1X10^6 mouse splenocytes were surface co-stained with FITC Anti-Mouse CD3 and 0.5 ug CoraLite® Plus 750 Anti-Mouse CD103 (CL750-65047, Clone: 2E7) or 0.5 ug Isotype Control. Cells were not fixed.

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Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65047 targets CD103 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL) 相同性解析による交差性が予測される生物種
Full Name integrin alpha E, epithelial-associated
GenBank accession numberBC150690
Gene symbol CD103
Gene ID (NCBI) 16407
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).

Protocols

Product Specific Protocols
FC protocol for CL Plus 750 CD103 antibody CL750-65047Download protocol
Standard Protocols
Click here to view our Standard Protocols