FITC Plus Anti-Human CD163 (GHI/61)
CD163 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
FC
Conjugate
FITC Plus Fluorescent Dye
CloneNo.
GHI/61
Cat no : FITC-65169
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | human PBMCs |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
FITC-65169 targets CD163 in FC applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Glycoprotein preparation from hairy cell leukemia spleen 相同性解析による交差性が予測される生物種 |
Full Name | CD163 molecule |
Calculated molecular weight | 1156 aa, 125 kDa |
GenBank accession number | BC051281 |
Gene symbol | CD163 |
Gene ID (NCBI) | 9332 |
Conjugate | FITC Plus Fluorescent Dye |
Excitation/Emission maxima wavelengths | 495 nm / 524 nm |
Form | Liquid |
Purification Method | Purified by protein-A affinity chromatography |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).
Protocols
Product Specific Protocols | |
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FC protocol for FITC Plus CD163 antibody FITC-65169 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |