Validation Data Gallery
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Tested Applications
| Positive IF-P detected in | mouse cerebellum tissue, rat cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66394 targets Calbindin-D28k in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Calbindin-D28k fusion protein Ag5861 相同性解析による交差性が予測される生物種 |
| Full Name | calbindin 1, 28kDa |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC006478 |
| Gene Symbol | Calbindin |
| Gene ID (NCBI) | 793 |
| RRID | AB_2934459 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P05937 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Calbindin-D28k antibody CL488-66394 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |