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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Calbindin-D28k Monoclonal antibody

Calbindin-D28k Monoclonal Antibody for IF-Fro

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-Fro

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F8B9

Cat no : CL488-66394

Synonyms

Calbindin, CALB1, Calbindin D28, calbindin 1, 28kDa, CALB

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using CoraLite® Plus 488-conjugated Calbindin-D28k Monoc (CL488-66394)

    IF Staining of mouse cerebellum using CL488-66394

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using CoraLite® Plus 488-conjugated Calbindin-D28k Monoc (CL488-66394)

    IF Staining of mouse cerebellum using CL488-66394

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat cerebellum tissue using CoraLite® Plus 488-conjugated Calbindin-D28k Monoc (CL488-66394)

    IF Staining of rat cerebellum using CL488-66394

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat cerebellum tissue using CoraLite® Plus 488-conjugated Calbindin-D28k Monoc (CL488-66394)

    IF Staining of rat cerebellum using CL488-66394

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using CoraLite® Plus 488-conjugated Calbindin-D28k Monoc (CL488-66394)

    IF Staining of mouse cerebellum using CL488-66394

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using CoraLite® Plus 488-conjugated Calbindin-D28k Monoc (CL488-66394)

    IF Staining of mouse cerebellum using CL488-66394

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200.

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Tested Applications

Positive IF-Fro detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66394 targets Calbindin-D28k in IF-Fro applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calbindin-D28k fusion protein Ag5861 相同性解析による交差性が予測される生物種
Full Name calbindin 1, 28kDa
Calculated molecular weight 30 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC006478
Gene symbol Calbindin
Gene ID (NCBI) 793
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Calbindin-D28k antibody CL488-66394Download protocol
Standard Protocols
Click here to view our Standard Protocols