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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using CoraLite®594-conjugated Calbindin-D28k Monoclonal (CL594-66394)

    IF Staining of mouse cerebellum using CL594-66394

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CL594-66394 (Calbindin-D28k antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using CoraLite®594-conjugated Calbindin-D28k Monoclonal (CL594-66394)

    IF Staining of mouse cerebellum using CL594-66394

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CL594-66394 (Calbindin-D28k antibody) at dilution of 1:100.

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CoraLite®594-conjugated Calbindin-D28k Monoclonal antibody

Calbindin-D28k Monoclonal Antibody for IF-P
Cat No. CL594-66394
Clone No.1F8B9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

CAB27, CALB, CALB1, Calbindin, calbindin 1, 28kDa, Calbindin D28, D 28K

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66394 targets Calbindin-D28k in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calbindin-D28k fusion protein Ag5861 相同性解析による交差性が予測される生物種
Full Name calbindin 1, 28kDa
Calculated molecular weight 30 kDa
GenBank accession numberBC006478
Gene Symbol Calbindin
Gene ID (NCBI) 793
RRIDAB_2883575
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05937
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

Protocols

Product Specific Protocols
IF protocol for CL594 Calbindin-D28k antibody CL594-66394Download protocol
Standard Protocols
Click here to view our Standard Protocols