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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®488-conjugated Cytokeratin 14 Monoclonal antibody

Cytokeratin 14 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, IF-P

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2G1E2

Cat no : CL488-60320

Synonyms

KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using CoraLite®488-conjugated Cytokeratin 14 Monoclonal (CL488-60320)

    IF Staining of mouse skin using CL488-60320

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using CoraLite®488-conjugated Cytokeratin 14 Monoclonal (CL488-60320)

    IF Staining of mouse skin using CL488-60320

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite®488-conjugated Cytokeratin 14 Monoclonal (CL488-60320)

    IF Staining of A431 using CL488-60320

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CL488-60320 (Cytokeratin 14 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using CoraLite®488-conjugated Cytokeratin 14 Monoclonal (CL488-60320)

    IF Staining of HaCaT using CL488-60320

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite®488-conjugated Cytokeratin 14 Monoclonal (CL488-60320)

    IF Staining of A431 using CL488-60320

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®488-conjugated Cytokeratin 14 Monoclonal (CL488-60320)

    IF Staining of HepG2 using CL488-60320

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inmouse skin tissue
Positive IF/ICC detected inA431 cells, HepG2 cells, HaCaT cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60320 targets Cytokeratin 14 in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 14 fusion protein Ag17559 相同性解析による交差性が予測される生物種
Full Name keratin 14
Calculated molecular weight 472 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC002690
Gene symbol Cytokeratin 14
Gene ID (NCBI) 3861
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths491 nm / 516 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

eratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

Protocols

Product Specific Protocols
IF protocol for CL488 Cytokeratin 14 antibody CL488-60320Download protocol
Standard Protocols
Click here to view our Standard Protocols