CoraLite®594-conjugated Cytokeratin 14 Monoclonal antibody
Cytokeratin 14 Monoclonal Antibody for IF/ICC, IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF/ICC, IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2G1E2
Cat no : CL594-60320
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse skin tissue |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-60320 targets Cytokeratin 14 in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Cytokeratin 14 fusion protein Ag17559 相同性解析による交差性が予測される生物種 |
| Full Name | keratin 14 |
| Calculated molecular weight | 472 aa, 52 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC002690 |
| Gene symbol | Cytokeratin 14 |
| Gene ID (NCBI) | 3861 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
eratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Cytokeratin 14 antibody CL594-60320 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
