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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Cytokeratin 14 Monoclonal antibody

Cytokeratin 14 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2G1E2

Cat no : CL647-60320

Synonyms

KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using CoraLite® Plus 647-conjugated Cytokeratin 14 Monoc (CL647-60320)

    IF Staining of mouse skin using CL647-60320

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using CoraLite® Plus 647 Cytokeratin 14 antibody (CL647-60320, Clone: 2G1E2 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using CoraLite® Plus 647-conjugated Cytokeratin 14 Monoc (CL647-60320)

    IF Staining of mouse skin using CL647-60320

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using CoraLite® Plus 647 Cytokeratin 14 antibody (CL647-60320, Clone: 2G1E2 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 647-conjugated Cytokeratin 14 Monoc (CL647-60320)

    FC experiment of A431 using CL647-60320

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Cytokeratin 14 (CL647-60320, Clone:2G1E2) (red), or 0.8 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse skin tissue
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-60320 targets Cytokeratin 14 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 14 fusion protein Ag17559 相同性解析による交差性が予測される生物種
Full Name keratin 14
Calculated molecular weight 472 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC002690
Gene symbol Cytokeratin 14
Gene ID (NCBI) 3861
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Cytokeratin 14 antibody CL647-60320Download protocol
FC protocol for CL Plus 647 Cytokeratin 14 antibody CL647-60320Download protocol
Standard Protocols
Click here to view our Standard Protocols