☆ 3/31(月)まで!二次抗体 & FlexAbleが30%オフ! >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cytokeratin 19 Monoclonal antibody

Cytokeratin 19 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL488-60187
Clone No.3G1E4

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, IF-P, FC (Intra)

CK19, CK 19, KRT19, Cytokeratin-19, Cytokeratin,Keratin

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60187 targets Cytokeratin 19 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag7407 相同性解析による交差性が予測される生物種
Full Name keratin 19
Calculated molecular weight 44 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC002539
Gene Symbol Cytokeratin 19
Gene ID (NCBI) 3880
RRIDAB_2883114
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08727
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19. KRT17 is used as a marker for trauma.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 19 antibody CL488-60187Download protocol
FC protocol for CL Plus 488 Cytokeratin 19 antibody CL488-60187Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...