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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Cytokeratin 19 Monoclonal antibody

Cytokeratin 19 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3G1E4

Cat no : CL647-60187

Synonyms

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human oesophagus cancer tissue using CoraLite® Plus 647-conjugated Cytokeratin 19 Monoc (CL647-60187)

    IF Staining of human oesophagus cancer using CL647-60187

    Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-60187, Clone: 3G1E4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite® Plus 647-conjugated Cytokeratin 19 Monoc (CL647-60187)

    IF Staining of human breast cancer using CL647-60187

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-60187, Clone: 3G1E4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite® Plus 647-conjugated Cytokeratin 19 Monoc (CL647-60187)

    IF Staining of human breast cancer using CL647-60187

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-60187, Clone: 3G1E4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated Cytokeratin 19 Monoc (CL647-60187)

    FC experiment of HeLa using CL647-60187

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Cytokeratin 19 (CL647-60187, Clone:3G1E4) (red), or 0.8 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inhuman breast cancer tissue, human oesophagus cancer tissue
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-60187 targets Cytokeratin 19 in IF-P, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag7407 相同性解析による交差性が予測される生物種
Full Name keratin 19
Calculated molecular weight 44 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC002539
Gene symbol Cytokeratin 19
Gene ID (NCBI) 3880
RRIDAB_2920244
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Cytokeratin 19 antibody CL647-60187Download protocol
FC protocol for CL Plus 647 Cytokeratin 19 antibody CL647-60187Download protocol
Standard Protocols
Click here to view our Standard Protocols