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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated Cytokeratin 19 Monoclonal antibody

Cytokeratin 19 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3G1E4

Cat no : CL555-60187

Synonyms

CK19, CK 19, KRT19, Cytokeratin-19, Cytokeratin,Keratin

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite®555-conjugated Cytokeratin 19 Monoclonal (CL555-60187)

    IF Staining of human breast cancer using CL555-60187

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite®555-conjugated Cytokeratin 19 Monoclonal (CL555-60187)

    IF Staining of human breast cancer using CL555-60187

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human oesophagus cancer tissue using CoraLite®555-conjugated Cytokeratin 19 Monoclonal (CL555-60187)

    IF Staining of human oesophagus cancer using CL555-60187

    Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human oesophagus cancer tissue using CoraLite®555-conjugated Cytokeratin 19 Monoclonal (CL555-60187)

    IF Staining of human oesophagus cancer using CL555-60187

    Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated Cytokeratin 19 Monoclonal (CL555-60187)

    FC experiment of HeLa using CL555-60187

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Cytokeratin 19 (CL555-60187, Clone:3G1E4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inhuman breast cancer tissue, human oesophagus cancer tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-60187 targets Cytokeratin 19 in IF-P, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag7407 相同性解析による交差性が予測される生物種
Full Name keratin 19
Calculated molecular weight 44 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC002539
Gene symbol Cytokeratin 19
Gene ID (NCBI) 3880
RRIDAB_2919658
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.

Protocols

Product Specific Protocols
IF protocol for CL555 Cytokeratin 19 antibody CL555-60187Download protocol
FC protocol for CL555 Cytokeratin 19 antibody CL555-60187Download protocol
Standard Protocols
Click here to view our Standard Protocols