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ChromoTek GFP-Trap® Magnetic Particles M-270 Kit

GFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Magnetic Particles M-270.

Specificity

GFP and GFP derivates

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic Particles M-270

Cat no : gtdk

Synonyms

GFP Trap Magnetic Particles M-270, GFP-Trap Magnetic Particles M-270, GFP-Trap Magnetic Particles M-270 kit, GFP-Trap® Magnetic Particles M-270 kit, gtdk, gtdk-20, gfp, GFP tag, GFP-tag, GFP-Trap® Magnetic Particles M-270, gtdk-20

Validation Data Gallery

  • Immunoprecipitation (IP) of GFP with GFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound.

    Immunoprecipitation (IP) of GFP with GFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound.

  • Immunoprecipitation (IP) of GFP with GFP-Trap Magnetic Particles M-270: Coomassie and Western blot (I: Input, FT: Flow-Through, B: Bound).

    Immunoprecipitation (IP) of GFP with GFP-Trap Magnetic Particles M-270: Coomassie and Western blot (I: Input, FT: Flow-Through, B: Bound).

  • Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270.

    Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270.

Product Information

GFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Magnetic Particles M-270.

DescriptionThe GFP-Trap Magnetic Particles M-270 Kit contains GFP-Trap Magnetic Particles M-270, lysis, wash, and elution buffers for efficient immunoprecipitation of GFP-fusion proteins and their interacting factors.

• IP and Co-IP of GFP-tagged proteins without size limitation

• Magnetic separation with easy and efficient washing of GFP-Trap Magnetic Particles M-270

• Automation and high throughput applications

• No heavy & light antibody chains, short incubation (5-30 min)

• Extraordinary binding, also under harsh conditions

• Very high affinity (KD=1 pM) to bind even low abundant proteins

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetAcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity2-2.8 μg of recombinant GFP per 25 μL bead slurry
ConjugateMagnetic Particles M-270, size: 2.8 µm
high throughput-compatible
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility10 mM DTT, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 0.2 % SDS, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 1 pM
Compatibility with mass spectrometryThe GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827592
Storage BufferPBS with 0.09% sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution
GFP-Trap® Magnetic Particles M-27020 reactions (500 µL)

Publications

ApplicationTitle
IP

Nat Commun

Remodeling the cellular stress response for enhanced genetic code expansion in mammalian cells

Authors - Mikhail E Sushkin
View Article
IP

EMBO Rep

Foxd3 controls heterochromatin-mediated repression of repeat elements and 2-cell state transcription.

Authors - Deepika Puri
View Article
IF

PLoS Biol

Genome-wide functional analysis reveals key roles for kinesins in the mammalian and mosquito stages of the malaria parasite life cycle

Authors - Mohammad Zeeshan
View Article
IP

Exp Cell Res

YBX2 modulates mRNA stability via interaction with YTHDF2 in endometrial cancer cells

Authors - Ying Cai
View Article
IP

Cell Rep

Dual agonistic and antagonistic roles of ZC3H18 provide for co-activation of distinct nuclear RNA decay pathways

Authors - Patrik Polák
View Article
IP

Cell Mol Life Sci

Inhibition of autocrine HGF maturation overcomes cetuximab resistance in colorectal cancer

Authors - Vivian Truong Jones
View Article