Validation Data Gallery
Product Information
Kit for IP/ Co-IP of GFP-fusion proteins & sample preparation for mass spectrometry (MS)
| Description | The iST GFP-Trap® Kit enables researchers to process GFP-fusion proteins and their interacting partners for Mass Spectrometry analysis by including the ChromoTek GFP-Trap for immunoprecipitation/Co-IP of GFP-fusion proteins and the PreOmics iST buffers and cartridges required for bottom-up proteomic sample preparation. This robust method yields purified peptides while dramatically reducing contamination and sample loss. Each kit accommodates up to eight samples and includes pull-down reagent for controls:
• Reliable dentification of GFP-fusion proteins & interacting partners by MS • Identification of even low abundant/ low expressed GFP-fusion proteins • Superior processing of IP samples into clean peptide mixtures • Intuitive protocol for reproducible results |
| Applications | IP, MS |
| Specificity/Target | AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP For the complete list, please click here: Fluorescent protein specificity table |
| Binding capacity | 25-30 µg of recombinant GFP per 25 µL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 1 pM |
| Compatibility with mass spectrometry | The GFP-Trap® is optimized for on-bead digestion. |
| Storage Buffer | 20% ethanol |
| Storage Condition | Kit is shipped at ambient temperature. Upon receipt store GFP-Trap at 4°C and Digest at -20°C |
Kit components
| Component | Description |
|---|---|
| GFP-Trap Agarose | 8 reactions plus 2 controls |
| Digest | Enzyme Trypsin-mix to digest proteins. |
| Resuspend buffer | Protease reconstitution buffer for enzymes. |
| Lyse buffer | Denature, reduce and alkylate proteins. |
| Stop solution | Stop the enzymatic activity. |
| Wash 1 buffer | Clean up peptides from hydrophobic contaminants. |
| Wash 2 buffer | Clean up peptides from hydrophilic contaminants. |
| Elution buffer | Elute the peptides from the cartridge. |
| LC-Load load | Load peptides on reversed-phase LC-MS column. |
| Cartridges | Cartridge for 1 to 100 µg protein starting material. |
| Waste tubes | Tube for collecting waste after washing steps. |
| Collection tubes | Tube for collecting peptides after elution. |
| Adapters | Enables placing a cartridge into a tube. |
| Caps | Cap to optionally close the cartridge’s bottom. |
Documentation
| SDS |
|---|
| SDS iST Kits (grouped) EtOH (EN)-CTK |
| Datasheet |
|---|
| iST GFP-Trap® IP-MS Sample Preparation Kit Datasheet |
| Application notes |
|---|
| iST GFP-Trap Kit application note (PDF) |
| Elution of GFP-fusion protein from the GFP-Trap |
| GFP-Trap Specificity |
|---|
| Fluorescent protein specificity table (PDF) |
Publications
| Application | Title |
|---|---|
J Biol Chem Phagocytosis mediated by the human granulocyte receptor CEACAM3 is limited by the receptor-type protein tyrosine phosphatase PTPRJ. | |
Life Sci Alliance Divergent Plasmodium kinases drive MTOC, kinetochore and axoneme organisation in male gametogenesis | |
J Clin Invest C16ORF70/MYTHO promotes healthy aging in C.elegans and prevents cellular senescence in mammals |