ChromoTek Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, for 2x Cys conjugation [CTK0101]
Monovalent, recombinant secondary single domain antibody to human IgG and rabbit IgG: alpaca monoclonal Nanobody, Fc- specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides
Applications
IF, WB, FC
Reactivity
Rabbit, Human, Macaque
Host
Alpaca
Conjugate
2x Cys
Cat no : srbGCys2-1
Synonyms
Validation Data Gallery
Product Information
Monovalent, recombinant secondary single domain antibody to human IgG and rabbit IgG: alpaca monoclonal Nanobody, Fc- specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides
| Applications | IF, WB, FC |
| Host | Alpaca |
| Reactivity | Rabbit, Human, Macaque |
| Specificity/Target | Fc-fragment of human IgG and rabbit IgG (co-reactivity) |
| Conjugate | N- and C-terminal cysteine conjugation with thiol-reactive reagents |
| Molecular weight | 14.7 kDa |
| Extinction coefficient (280 nm) | 24,200 M-1*cm-1 |
| Type | Nano-Secondary®, secondary Nanobody (VHH) |
| Format | Alpaca single domain antibody, monovalent |
| Cross-reactivity | No cross-reactivity to goat, guinea pig, mouse, rat, and sheep serum Cross-reactivity to macaque serum |
| Immunogen | Purified rabbit IgG |
| Clonality | Monoclonal Nanobody |
| Clone | CTK0101 |
| Affinity (KD) | KD = 0.2 nM |
| RRID | AB_2864261 |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 1 mM TCEP Preservative: 0.09 % Sodium azide |
| Storage Condition | Shipped on dry ice. Store at -20°C/-4°F. Avoid freeze-thaw cycles. |
| Size | 500 µg |
Publications
| Application | Title |
|---|---|
Atherosclerosis A comparative gene expression matrix in Apoe-deficient mice identifies unique and atherosclerotic disease stage-specific gene regulation patterns in monocytes and macrophages | |