ChromoTek Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 568 [CTK0101, CTK0102]
Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.
Applications
IF, WB, FC
Conjugate
Alexa Fluor® 568
Host
Alpaca
Reactivity
Rabbit, Human, Macaque
Cat no : srbAF568-1
Synonyms
Validation Data Gallery
Product Information
Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.
| Description | Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH reagent uses a novel class of anti-rabbit IgG and anti-human IgG specific antibodies. This secondary antibody product consists of Nanobodies that bind to rabbit IgG and human IgG with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below. |
| Applications | IF, WB, FC |
| Host | Alpaca |
| Species Reactivity | Rabbit, Human, Macaque No cross-reactivity to mouse, rat, sheep, goat, and guinea pig IgG |
| Conjugate | Alexa Fluor® 568 |
| Physical State | Liquid |
| Suggested Dilution | Immunofluorescence 1:1,000 Super-resolution microscopy 1:1,000 Western blot 1:1,000 |
| Purification Method | Recombinant expression, affinity purification IMAC |
| Type | Mixture of 2 monoclonal Nanobodies |
| Class | Recombinant |
| Affinity (KD) | CTK0101: KD = 0.2 nM, CTK0102: KD = 1.2 nM |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
| Storage Condition | Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles. |
| RRID | AB_2827586 |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
| SDS |
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| SDS_Nano-Secondary® alpaca anti-human IgG, anti-rabbit IgG, Alexa Fluor® 568 |
| Protocol |
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| Protocol_Nano-Secondary® alpaca anti-human IgG, anti-rabbit IgG, Alexa Fluor® 568 |
| Application note |
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| One-step incubation with Nano-Secondaries (PDF) |
| Blocking recommendation for immunostaining with Nano-Secondaries (PDF) |
| Brochure |
|---|
| Chromotek nanobodies brochure (PDF) |
Publications
| Application | Title |
|---|---|
Sci Adv Mutation of SLC7A14 causes auditory neuropathy and retinitis pigmentosa mediated by lysosomal dysfunction. | |
Gene Ther A new protocol for whole-brain biodistribution analysis of AAVs by tissue clearing, light-sheet microscopy and semi-automated spatial quantification | |
Neuroscience Translocator Protein Regulate Polarization Phenotype Transformation of Microglia after Cerebral Ischemia-reperfusion Injury | |
Front Bioeng Biotechnol Maintenance of multipotency of bone marrow mesenchymal stem cells on poly(ε-caprolactone) nanoneedle arrays through the enhancement of cell-cell interaction | |
Phytomedicine Hesperidin inhibits lung fibroblast senescence via IL-6/STAT3 signaling pathway to suppress pulmonary fibrosis | |
EMBO Rep Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Davide (Verified Customer) (06-13-2023) | The secondary antibody worked very well and the spatial resolution was great on 3d human primary cell cultures. On the image the yellow is the antibody used. The primary antibody was a ck19 rabbit.
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