Biotin-conjugated NeuN Monoclonal antibody
NeuN Monoclonal Antibody for IHC
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
IHC
Conjugate
Biotin
CloneNo.
3A4C1
Cat no : Biotin-66836
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-66836 targets NeuN in IHC applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NeuN fusion protein Ag28016 相同性解析による交差性が予測される生物種 |
| Full Name | hexaribonucleotide binding protein 3 |
| GenBank accession number | NM_001082575 |
| Gene symbol | NeuN |
| Gene ID (NCBI) | 146713 |
| RRID | AB_2883076 |
| Conjugate | Biotin |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin NeuN antibody Biotin-66836 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
