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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated NeuN Monoclonal antibody

NeuN Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A4C1

Cat no : CL488-66836

Synonyms

Neuronal nuclei antigen, NeuN antigen, HRNBP3, FOX3, Fox-1 homolog C

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated NeuN Monoclonal anti (CL488-66836)

    IF Staining of mouse brain using CL488-66836

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66836 (NeuN antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using CoraLite® Plus 488-conjugated NeuN Monoclonal anti (CL488-66836)

    IF Staining of mouse cerebellum using CL488-66836

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite® Plus 488 NeuN antibody (CL488-66836, Clone: 3A4C1 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated NeuN Monoclonal anti (CL488-66836)

    IF Staining of mouse brain using CL488-66836

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66836 (NeuN antibody), at dilution of 1:100.

  • Flow cytometry (FC) experiment of U-87 MG cells using CoraLite® Plus 488-conjugated NeuN Monoclonal anti (CL488-66836)

    FC experiment of U-87 MG using CL488-66836

    1X10^6 U-87 MG cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NeuN (CL488-66836, Clone:3A4C1) (red), or 0.4 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite® Plus 488-conjugated NeuN Monoclonal anti (CL488-66836)

    FC experiment of SH-SY5Y using CL488-66836

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NeuN (CL488-66836, Clone:3A4C1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF-P detected inmouse cerebellum tissue, mouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL488-66836 targets NeuN in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NeuN fusion protein Ag28016 相同性解析による交差性が予測される生物種
Full Name hexaribonucleotide binding protein 3
GenBank accession numberNM_001082575
Gene symbol NeuN
Gene ID (NCBI) 146713
RRIDAB_2883386
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NeuN antibody CL488-66836Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Exp Eye Res

Geranylgeranylacetone-induced heat shock protein70 expression reduces retinal ischemia-reperfusion injury through PI3K/AKT/mTOR signaling

Authors - Lirong Zhang
View Article
mouse

bioRxiv

2P-NucTag: on-demand phototagging for molecular analysis of functionally identified cortical neurons

Authors - Jingcheng Shi
View Article