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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated Phospho-Histone H3 (Ser10) Monoclonal antibody

Phospho-Histone H3 (Ser10) Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4C7G2

Cat no : CL647-66863

Synonyms

H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, Phospho-Histone H3 (Ser10)

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL647-66863

    FC experiment of HeLa using CL647-66863

    1X10^6 HeLa cells untreated (dashed lines) or treated with nocodazole (red) were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Human Phospho-Histone H3 (Ser10) (CL647-66863, Clone:4C7G2), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • FC experiment of HeLa using CL647-66863

    FC experiment of HeLa using CL647-66863

    1X10^6 unstimulated or 20 µM Nocodazole treated 24 hours HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Phospho-Histone H3 (Ser10) (CL647-66863, Clone:4C7G2). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Tested Applications

Positive FC detected in20 µM Nocodazole treated 24 hours HeLa cells, nocodazole treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66863-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL647-66863 targets Phospho-Histone H3 (Ser10) in FC (Intra) applications and shows reactivity with Human, rat, mouse, pig samples.

Tested Reactivity Human, rat, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Calculated molecular weight 15 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberNM_003529
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.