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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated Phospho-Histone H3 (Ser10) Monoclonal antibody

Phospho-Histone H3 (Ser10) Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4C7G2

Cat no : CL647-66863

Synonyms

H3 S10, H3 Ser10, H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, H3S10, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone, histone cluster 1, H3a, Histone H3, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, PHH3, Phospho-Histone H3 (Ser10), Phospho-Histone H3 Ser10

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 647-conjugated Phospho-Histone H3 ( (CL647-66863)

    IF Staining of HeLa using CL647-66863

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 Phospho-Histone H3 (Ser10) antibody (CL647-66863, Clone: 4C7G2 ) at dilution of 1:200, Beta Tubulin antibody (80713-1-RR, Clone: 2O13, green).

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66863 targets Phospho-Histone H3 (Ser10) in IF/ICC applications and shows reactivity with Human, rat, mouse, pig samples.

Tested Reactivity Human, rat, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Calculated molecular weight 15 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberNM_003529
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Phospho-Histone H3 (Ser10) antibody CL647-66863Download protocol
Standard Protocols
Click here to view our Standard Protocols