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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Sestrin 2 Monoclonal (CL488-66297)

    IF Staining of HeLa using CL488-66297

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Sestrin 2 antibody (CL488-66297, Clone: 1A1A4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated Sestrin 2 Monoclonal (CL488-66297)

    FC experiment of HeLa using CL488-66297

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Sestrin 2 (CL488-66297, Clone:1A1A4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Sestrin 2 Monoclonal antibody

Sestrin 2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66297
Clone No.1A1A4

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66297 targets Sestrin 2 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Sestrin 2 fusion protein Ag20638 相同性解析による交差性が予測される生物種
Full Name sestrin 2
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC013304
Gene Symbol SESN2/Sestrin 2
Gene ID (NCBI) 83667
RRIDAB_3084231
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP58004
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Sestrin 2 antibody CL488-66297Download protocol
FC protocol for CL Plus 488 Sestrin 2 antibody CL488-66297Download protocol
Standard Protocols
Click here to view our Standard Protocols