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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Sestrin 2 Monoclonal antibody

Sestrin 2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A1A4

Cat no : CL594-66297

Synonyms

DKFZp761M0212, DKFZp761M02121, HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Sestrin 2 Monoclonal antib (CL594-66297)

    IF Staining of HeLa using CL594-66297

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 Sestrin 2 antibody (CL594-66297, Clone: 1A1A4 ) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66297 targets Sestrin 2 in IF/ICC applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Sestrin 2 fusion protein Ag20638 相同性解析による交差性が予測される生物種
Full Name sestrin 2
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC013304
Gene symbol SESN2/Sestrin 2
Gene ID (NCBI) 83667
RRIDAB_2919975
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

Protocols

Product Specific Protocols
IF protocol for CL594 Sestrin 2 antibody CL594-66297Download protocol
Standard Protocols
Click here to view our Standard Protocols