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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite® Plus 488-conjugated transgelin/SM22 Poly (CL488-10493)

    IF Staining of mouse heart using CL488-10493

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite® Plus 488 transgelin/SM22 antibody (CL488-10493) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated transgelin/SM22 Polyclonal antibody

transgelin/SM22 Polyclonal Antibody for IF-P
Cat No. CL488-10493

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

SM22, transgelin, Smooth muscle protein 22-alpha, SM22-alpha, SM22-a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10493 targets transgelin/SM22 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen transgelin/SM22 fusion protein Ag0764 相同性解析による交差性が予測される生物種
Full Name transgelin
Calculated molecular weight 22 kDa
Observed molecular weight 19-22 kDa
GenBank accession numberBC004927
Gene Symbol transgelin/SM22
Gene ID (NCBI) 6876
RRIDAB_2918981
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01995
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transgelin family is a group of proteins that belong to 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgelin 1 protein. It can cross-react with other two transgelins based on the sequence similarity.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 transgelin/SM22 antibody CL488-10493Download protocol
Standard Protocols
Click here to view our Standard Protocols